scatterplots and pearson correlation coefficients (r values) between proteins and mrna expression ratios (GraphPad Software Inc)
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Scatterplots And Pearson Correlation Coefficients (R Values) Between Proteins And Mrna Expression Ratios, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Integration of proteomic and transcriptomic profiles reveals multiple levels of genetic regulation of salt tolerance in cotton"
Article Title: Integration of proteomic and transcriptomic profiles reveals multiple levels of genetic regulation of salt tolerance in cotton
Journal: BMC Plant Biology
doi: 10.1186/s12870-018-1350-1
Figure Legend Snippet: Schematic overview of proteomic and transcriptomic data generation and analysis workflow. Step 1: differential expression analysis of proteomic and Step 2: differential expression analysis of mRNA-seq data. Step 3: AS (alternative splicing) analysis using TopHat software and alternatively spliced differentially expressed genes (AS-DEGs) were obtained. Step 4: mapping of all proteins from the proteomic to mRNA-seq data and output of the two target groups. Among them, Group A contains translation repression and/or transcriptional degradation targets (contains differentially expressed genes and proteins whose levels were unchanged, both genes and proteins that were differentially expressed or no proteomic data). Group B contains potential targets of miRNA translational repression (differentially abundant proteins whose mRNA did not change or no mRNA data). Step 5: genome-wide analysis of differentially expressed conserved and novel miRNAs in both cotton genotypes under control and salt stress conditions. Step 6: psRNATarget analysis of both groups against miRNAs DE in the opposite direction combined with Group A and B data
Techniques Used: Quantitative Proteomics, Alternative Splicing, Software, Genome Wide, Control
Figure Legend Snippet: Hierarchical clustering analysis of mRNA-seq and proteomic data based on expression data. Heatmap of the proteins and mRNA expression ratios that have the same ( a , c ) or opposite ( b , d ) change tendencies after 4 h and 24 h of salt treatment, respectively. The expression profiles shown in the left and right panels are based on standardized log 2 ratio values. The minimum and maximum displayed log 2 ratios are ±3 for the transcriptomic data and ± 0.5 for the proteomics data. Black represents no significant change in expression. The KEGG pathway of genes/proteins showing significance are represented by various symbols behind the gene ID
Techniques Used: Expressing